Coding

Part:BBa_K4200000:Design

Designed by: Tushar Mishra   Group: iGEM22_MIT_MAHE   (2022-10-10)


Multivalent Adhesion Molecule 7


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 126
    Illegal BamHI site found at 1141
    Illegal XhoI site found at 2521
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 46
    Illegal NgoMIV site found at 867
    Illegal NgoMIV site found at 1317
    Illegal AgeI site found at 1264
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 304


Design Notes

The sequence derived from Vibrio parahaemolyticus genome was codon optimized for Escherichia coli using the relevant tool in Benchling.

Source

Vibrio parahaemolyticus genome, synthetic

References

A. Krachler, H. Ham and K. Orth, "Outer membrane adhesion factor multivalent adhesion molecule 7 initiates host cell binding during infection by Gram-negative pathogens", Proceedings of the National Academy of Sciences, vol. 108, no. 28, pp. 11614-11619, 2011. Available: 10.1073/pnas.1102360108 [Accessed 10 October 2022].